Transposon-generated ‘knock-out’ and ‘knock-in’ gene-targeting constructs for use in mice
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چکیده
منابع مشابه
Transposon-generated ‘knock-out’ and ‘knock-in’ gene-targeting constructs for use in mice
The conventional technique for targeted mutation of mouse genes entails placing a genomic DNA fragment containing the gene of interest into a vector for fine mapping, followed by cloning of two genomic arms around a selectable neomycin-resistance cassette in a vector containing thymidine kinase [1]; this generally requires 1-2 months of work for each construct. The single 'knock-out' construct ...
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ژورنال
عنوان ژورنال: Current Biology
سال: 1997
ISSN: 0960-9822
DOI: 10.1016/s0960-9822(06)00224-7